Journal: Cancer Cell International
Article Title: FAM111B promotes prostate cancer progression by inhibiting apoptosis via ATF3 suppression and MAPK pathway activation: a novel biomarker and therapeutic target
doi: 10.1186/s12935-025-04111-3
Figure Lengend Snippet: FAM111B is highly expressed in prostate cancer and strongly associated with poor prognostic survival. ( A ) The Venn diagram of the Red module, cell cycle, DNA replication, protein DNA complex, and Proteolysis-related genes. ( B-D ) FAM111B expression levels in TCGA in normal versus tumor patients, in cancer versus paraneoplastic in the same patient, and in normal versus patients with various stages of the disease. ( E ) ROC curve for Subjects in the PCa Cohort in TCGA. ( F-G ) FAM111B expression levels in GSE3325 in normal versus tumor patients and in normal versus patients with various stages of the disease. ( H ) ROC curve for Subjects in the PCa Cohort in GSE3325. ( I-K ) Association of FAM111B expression level with tumor analysis, Gleason score, and time to recurrence using chi-square analysis. ( L ) K-M curves for PCa pair column disease-free survival in TCGA. ( M ) One-way COX regression analysis and forest plot for the TOP 10 genes in the red module. ( N ) FAM111B relative mRNA expression levels in PrSC, RWPE1, LNCaP, DU145, PC3 cell lines. ( O-P ) WB of FAM111B and quantitative analysis relative protein expression levels in PrSC, RWPE1, LNCaP, DU145, PC3 cell lines. ( Q ) FAM111B relative expression in cancer and paracancerous tissues of 18 Wuhan University People’s Hospital clinical patients. ( R ) WB of FAM111B in cancer and paracancerous tissues of 18 Wuhan University People’s Hospital clinical patients. ( S ) FAM111B staining in cancer and para cancer IHC in 2 Wuhan University People’s Hospital patients
Article Snippet: The prostate epithelial cell line RWPE1 and PCa cell lines LNCaP, 22RV1, DU145, PC3, and VCaP were obtained from the American Type Culture Collection (ATCC).
Techniques: Expressing, Staining